Methylation-sensitive amplification polymorphism (MSAP) protocol to assess CpG and CpNpG methylation in the banana genome

Pattern of DNA cytosine methylation in Aeluropus littoralis during temperature stress

DNA methylation as an epigenetic mediator plays the important role in spatial and temporal gene regulation and ensures the stability and the plasticity of organism. In this investigation, methylation sensitive amplification polymorphism (MSAP) were assessed in CCGG sites on a halophytic plant, Aeluropuslittoralis in response to different temperature stresses including freezing...

Involvement of Cytosine DNA methylation in different developmental stages of Aeluropus littoralis

DNA methylation as epigenetic mark plays a key role in normal differential and developmental processes as well as in dynamic gene regulation at the genomic level. To assess DNA methylation pattern in different developmental stages of Aeluropus littoralis, methylation sensitive amplified polymorphism (MSAP) was used. Methylation and demethylation status at the CCGG recognition site were...

Methylation-sensitive amplification polymorphism analysis of fat and muscle tissues in pigs.

DNA methylation may be involved in regulating the expression of protein-coding genes, resulting in different fat and muscle phenotypes. Using a methylation-sensitive amplified polymorphism approach, we obtained 7423 bands by selective amplification of genomic DNA from six different fat depots and two heterogeneous muscle types from Duroc/Landrace/Yorkshire cross-bred pigs. The degrees of D...

Sensitive and quantitative universal Pyrosequencing methylation analysis of CpG sites.

MSAP markers and global cytosine methylation in plants

17 Methylation of DNA cytosines affects whether transposons are silenced and genes are 18 expressed, and is a major epigenetic mechanism whereby plants respond to 19 environmental change. Analyses of Methylation-Sensitive Amplification Polymorphism 20 (MS-AFLP or MSAP) have been often used to assess methyl-cytosine changes in 21 response to stress treatments and, more recently, in ecological st...